Manual ImmunochemicalNephelometric Assaysfor Serum ImmunoglobulinsIgG , IgA , and 1 gM lld 1
نویسندگان
چکیده
SubmitGregory J. Buffone and Susan A. Lewis, Clinical ters: Chemistry Laboratory, Children’s Hospital National Medical Center, ill Michigan Ave., N. W., Washington, DC 20010 Eva luaCarl R. Jolliff, Lincoln Clinic, Lincoln, NE tors: 68501 Harold Markowitz, Mayo Clinic, Rochester, MN 55901 Gabriel Virella, H. Hugh Fundenberg, Jose Munoz, Nick Burdash, and Patricia Buchner, Medical University of South Carolina, Charleston, SC 29403
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Manual procedures are presented for determining immunoglobulins IgG, IgA, and 1gM in human serum. The three immunoglobulins are quantitated, after reaction with specific ant sera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and 1gM. Results with the present system correlated ex...
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